-
평점
-
작성일 2009-02-11
-
추천
-
조회수 1456
|
|
|
|
|
|
한국어 초록 |
|
|
차가버섯 수용성 추출물이 정상 세포주 NIH3T3 mousenormal fibroblast cell 및 인체 종양 세포주 AGS human gastric cancer cell (위암),HCT-15 human colon cancer cell (대장암), Hep3B human hepatoma cancer cell(간암), MCF-7 human breast cancer cell (유방암), HeLa human cervical cancer cell (자궁경부암)에서 MTT (3-[4,5-dmethylthiazol-2-yl]-2,5-diphenyl tetrazoliumbromide) as-say 방법에 의한 세포 증식 억제와 암세포 증식억제의 기전 연구의 일환으로 apoptosis가 일어날 때
나타나는 DNA fragmentation을 agarose gelelectrophoresis 방법으로 검토하였다. 인체 종양 세포주의생육저해 효과가 발효 차가버섯 추출물이 비발효 차가버섯 추출물보다 강한 것으로 나타났다.
그러나 동일한 실험조건하에서 마우스 정상세포주 NIH3T3은 80% 이상의 생존율을 나타내어 정상 세포주에는 큰 영향을 미치지 않는 것으로 나타났다. 특히, 발효 및 비발효차가버섯 추출물에서 본 실험에 사용한 세포주 중에서 대장암 세포주 HCT-15에 대해 가장 세포 증식 억제효과가 뛰어났으며, 이러한효과는 첨가 농도 의존적 이였다.
발효 및 비발효 차가버섯 추출물에 의한 암세포 증식억제가 기전 연구로 apoptosis가 일어날 때나타나는 DNA fragmentation을 세포로부터 genomic DNA를 분리하여 agarose gelelectrophoresis 방법으로 조사한 결과, 정상세포인 NIH3T3 세포는 DNA fragmentation이 거의 일어나지않아 세포 생존율 결과와 유사한 경향을 보였으나, 특히 대장암 세포주인 HCT-15에서는 발효 차가버섯뿐만 아니라 비발효 차가버섯추출물에서도 DNA fragmentation이 많이 일어나는 것이 관찰되어 암세포 증식억제 효과가 높다는 결과를 뒷받침 해주고있다.
This study was performed to investigate the effect of the water-extract from non-fermented or fermented Chaga mushrooms (Inonotus obliquus) on the proliferation and apoptosis of the NIH3T3 mouse normal fibroblast cells and various human cancer cell lines including HCT-15 human colon carcinoma, AGS human gastric carcinoma, MCF-7 human breast adenocarcinoma, Hep3B human hepatocellular carcinoma and HeLa human cervical carcinoma using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay and DNA fragmentation. In an anti-cancer test using various human cancer cells, fermented Chaga mushroom extract showed higher antiproliferating effect than that of non-fermented Chaga mushroom extract. Mouse normal NIH3T3 cells were exhibited 80% above survival under fermented or non-fermented Chaga mushroom extract of various concentrations (0, 0.5 and 1㎎/㎖). Fermented Chaga mushroom extract significantly inhibited cell growth on HCT-15 cells in a dose-dependent manner. HCT-15 cells treated with non-fermented or fermented Chaga mushrooms extract produced a distinct oligonucleosomal ladder with different sizes of DNA fragments, a typical characteristic of cells undergoing apoptosis. These results suggest that fermented Chaga mushroom extract suppresses growth of HCT-15 human colon carcinoma cells through apoptosis. |
|
|
|
|
|
저자 키워드 |
|
|
|
|
|
Inonoius abliquus, NIH3T3, HCT-15, AGS, MCF-7, Hep3B, HeLa |
|
|
|
|
|
참고 문헌 정보 |
|
|
|
|
|
⊙ Burczyki, J., A. Gawron, M. Slotwinska, B. Smietana and K. Terminska. 1996. Antimitotic activity of aqueous extracts of Inonotus obliquus. Bull Chem. Farm 135, 306-309. ⊙ Cha, J. Y., B. S. Jun, K. S. Yoo, J. R. Hahm and Y.S. Cho. 2006. Fermented Chaga mushroom (Inonotus obliquus) effects on hypolipidemia and hepatoprotection in the Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Food Sci. Biotechnol. 15, 122-127. ⊙ Cha, J. Y., B. S. Jun, J. W. Kim, S. H. Park, C. H. Lee and Y. S. Cho. 2006. Hypoglycemic effects of fermented Chaga mushroom (Inontus obliquus) in the diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Food Sci. Biotechnol. 15, 739-745. ⊙ Cha, J, Y., B, S. Jeon, J. W. Park, J. C. Moon and Y. S. Cho. 2004. Effect of fermented compositions containing Inonotus obliquus with Houttuynia cordata on growth of human AGS gastric and HCT-15 colon cancer cells. J. Korean Soc. Appl. Biol. Chem. 47, 20 ⊙ Cha, J. Y., B. S. Jeon, C. H. Lee, K. S. Yoo, J. S. Moon and Y. S. Cho. 2005. Hypoglycemic and antioxidative effects of fermented Chaga mushroom (Inonotus obliquus) on streptozotocin-induced diabetic rats. J. Life Sci. 15, 809-818. ⊙ Cha, J. Y., B. S. Jeon, J. C. Moon, J. H. Yoo and Y. S. Cho. 2004. Cytotoxic effect of Inonotus obliquus composition in HCT-15 human colon cancer cells and AGS gastric cancer cells. J. Korean Soc. Food Sci. Nutr, 33, 633-638. ⊙ Dnizot, F. D, and L. Rita. 1986. Rapid colorimetric assay for cell growth and survival: Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 22, 271-277. ⊙ Goh, E. L. K., R. J. Pircher and P. E. Lobie. 1998. Growth hormone promotion of tibulin polymerization stabilized the microtubule network and protects against colchicin-induced apoptosis. Endocrinology 139, 4364-4372. ⊙ Ham, S. S., S. W. Oh, Y. K. Kim, K. S. Shin, K. Y. Chang and G. H. Chung. 2003. Antimutagenic and cytotoxic effects of ethanol extract from the Inonotus obliquus. J. Korean Soc.. Food Sci. Nutr. 32, 1088-1094. ⊙ Hwang, Y. J., H. K. Nam, M. J. Chang, G. W. Noh and S. H. Kim. 2003. Effect of Lentinus edodes and Pleurotus eryngii extracts on proliferation and apoptosis in human colon cancer cell lines, J. Korean Soc. Food Sci. Nutr. 32, 217-222. ⊙ Hwang, Y. J., G. W. Noh and S. H. Kim. 2003. Effect of Inonotus obliquus extracts on proliferation and caspase-3 activity in human gastro-intestinal cancer cell lines. Korean J. Nutr. 36, 18-23. ⊙ Kahlos, K., L. Kangas and R. Hitunen. 1987. Antitumor activity of some compounds and fractions from an n-hexane extract of Inonotus obliquus. Acta. Pharm. Fenn. 96, 33-40. ⊙ Kim, S. W. 1998. Studies on anti-microbial and anti-cancer function of polysaccharide extracted Ganoderma lucidum. J. Korean Soc. Food Sci. Nutr. 27, 1183-1188. ⊙ Kim, Y. 0., S. B. Han, H. W. Lee, H. J. Ahn, Y. D. Yoon, J. K. Jung, H. M. Kim and C. S. Shin. 2005. Immuno-stimulating effect of the crude-polysaccharide produced by submerged culture of Inonotus obliquus. Life Sciences 77, 2438-2456. ⊙ Lee, I. S. and A. Nishikawa. 2003. Polyzellus multiplex, a Korean wild mushroom, as a potent chemopreventive agent against stomach cancer. Life Sci. 73, 3225-3234. ⊙ Li, X., J. Rong, M. Wu and X. Zeng. 2003. Anti-tumor effect of polysaccharide from Grifola frondosa and its influence on immunological function. Zhong Yao Cai 26, 31-32. ⊙ Mizuno, T., C. Zhuang, K. Abe, H. Okamoto, T. Kiho, S. Ukai, S. Leclerc and L. Meijer. 1999. Antitumor and hypohlycemic activities of polysaccharides from the Sclerotia and Mycelia of Inonotus obliquus (Pers.. Fe.) PⅡ. (Aphyllophoromycetideae). Int. J. Me ⊙ Mizuno, T., K. Minato, H. Ito, M. Kawade, H. Terai and H. Tsuchida. 1999. Antitumor polysaccharide from the mycelium of liquid-cultured Agricus blazei Murrill. Biochem. Mol. Biol. Int. 47, 707-714. ⊙ Ng, M. L. and A. T. Yap. 2002. Inhibition of human colon carcinoma development by lentinan from shiitake mushroom (Lentinus edodes). J. Alern Comlement Med. 8, 581-589. ⊙ Shivrina, A. N. 1967. Chemical characteristics of compounds extracted from Inonotus obliquus. Chem. Abstr. 66, 17271-17279. ⊙ Wasser, S. P. 2002. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbiol. Biotechnol. 60, 258-274.
|
|
|
|